验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67394 targets RGS14 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RGS14 fusion protein Ag9477 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | regulator of G-protein signaling 14 |
| 别名 | Regulator of G-protein signaling 14, 1C1B2 |
| 计算分子量 | 566 aa, 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC014094 |
| 基因名称 | RGS14 |
| Gene ID (NCBI) | 10636 |
| RRID | AB_3672945 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43566 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RGS14 antibody CL488-67394 | Download protocol |
| FC protocol for CL Plus 488 RGS14 antibody CL488-67394 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |