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RGSL1 Polyclonal antibody

RGSL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25836-1-AP

Print datasheet

Synonyms

RGSL, RGSL1, RGSL2

验证数据展示

  • WB analysis of mouse brain using 25836-1-AP

    WB analysis of mouse brain using 25836-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25836-1-AP (RGSL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25836-1-AP

    IHC staining of human testis using 25836-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 25836-1-AP

    IHC staining of human testis using 25836-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25836-1-AP (RGSL1 Antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25836-1-AP targets RGSL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RGSL1 fusion protein Ag20269 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of G-protein signaling like 1
Synonyms RGSL, RGSL1, RGSL2
Calculated Molecular Weight 1076 aa, 126 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC142944
Gene Symbol RGSL1
Gene ID (NCBI) 353299
RRIDAB_2880263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA5PLK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGSL1, also named as RGSL and RGSL2, belongs to the RSG family. RGSL1 is involved in the G-protein signaling regulation with a suggested role in prostate carcinogenesis (PMID: 28662289). RGSL1 has 6 isoforms with the molecular mass of 47-63, 71 and 126 kDa. RGSL1 may interact with other markers in the development of breast cancer (PMID: 21135262).

实验方案

Product Specific Protocols
WB protocol for RGSL1 antibody 25836-1-APDownload protocol
IHC protocol for RGSL1 antibody 25836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols