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验证数据展示

  • IF Staining of HeLa using 82760-2-RR (same clone as 82760-2-PBS)

    IF Staining of HeLa using 82760-2-RR (same clone as 82760-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • WB analysis using 82760-2-RR (same clone as 82760-2-PBS)

    WB analysis using 82760-2-RR (same clone as 82760-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82760-2-RR (same clone as 82760-2-PBS)

    WB analysis of HEK-293 using 82760-2-RR (same clone as 82760-2-PBS)

    WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82760-2-RR

    Affinity and Kinetic Characterization of 82760-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

  • Featured Product
  • KD/KO验证

RHOA Recombinant antibody, PBS Only

RHOA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82760-2-PBS

产品说明书

CloneNo. 240880E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.

产品信息

82760-2-PBS targets RHOA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RHOA fusion protein Ag1141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ras homolog gene family, member A
别名 ARHA, ARH12, RHO12, Rho cDNA clone 12, h12
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC005976
基因名称 RHOA
Gene ID (NCBI) 387
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61586
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.