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验证数据展示

  • IF Staining of HeLa using 82760-2-RR

    IF Staining of HeLa using 82760-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 82760-2-RR

    WB analysis using 82760-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82760-2-RR

    WB analysis of HEK-293 using 82760-2-RR

    WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82760-2-RR

    Affinity and Kinetic Characterization of 82760-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

  • Featured Product
  • KD/KO验证

RHOA Recombinant antibody

RHOA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82760-2-RR

产品说明书

CloneNo. 240880E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, HEK-293 cells, HeLa cells, HL-60 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82760-2-RR targets RHOA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RHOA fusion protein Ag1141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ras homolog gene family, member A
别名 ARHA, ARH12, RHO12, Rho cDNA clone 12, h12
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC005976
基因名称 RHOA
Gene ID (NCBI) 387
RRIDAB_3670546
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

实验方案

Product Specific Protocols
WB protocol for RHOA antibody 82760-2-RRDownload protocol
IF protocol for RHOA antibody 82760-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols