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验证数据展示

  • IF Staining of HeLa using 82760-2-RR

    IF Staining of HeLa using 82760-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 82760-2-RR

    WB analysis using 82760-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82760-2-RR

    WB analysis of HEK-293 using 82760-2-RR

    WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82760-2-RR

    Affinity and Kinetic Characterization of 82760-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

  • Featured Product
  • KD/KO Validated

RHOA Recombinant antibody

RHOA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82760-2-RR

Print datasheet

CloneNo. 240880E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, HEK-293 cells, HeLa cells, HL-60 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82760-2-RR targets RHOA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RHOA fusion protein Ag1141 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ras homolog gene family, member A
Synonyms ARHA, ARH12, RHO12, Rho cDNA clone 12, h12
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005976
Gene Symbol RHOA
Gene ID (NCBI) 387
RRIDAB_3670546
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

实验方案

Product Specific Protocols
WB protocol for RHOA antibody 82760-2-RRDownload protocol
IF protocol for RHOA antibody 82760-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols