RIC8A Monoclonal antibody
RIC8A Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1E3B11
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66625-1-Ig targets RIC8A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | human |
| Immunogen | RIC8A fusion protein Ag1593 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Synonyms | Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC011821 |
| Gene Symbol | RIC8A |
| Gene ID (NCBI) | 60626 |
| RRID | AB_2881985 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NPQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| IHC protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| IF protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| IP protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies |
