验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
For other applications, we recommend the unconjugated version of this antibody, 66625-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66625 targets RIC8A in IF-P applications and shows reactivity with Human, Rat, Mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, Rat, Mouse |
| 免疫原 | RIC8A fusion protein Ag1593 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| 别名 | Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A |
| 计算分子量 | 60 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC011821 |
| 基因名称 | RIC8A |
| Gene ID (NCBI) | 60626 |
| RRID | AB_2919352 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NPQ8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RIC8A antibody CL488-66625 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |