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验证数据展示

  • WB analysis of A431 using 84861-5-RR

    WB analysis of A431 using 84861-5-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84861-5-RR

    WB analysis using 84861-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84861-5-RR

    IF Staining of A431 using 84861-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RIG-1/DDX58 antibody (84861-5-RR, Clone: 242424E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84861-5-RR

    Affinity and Kinetic Characterization of 84861-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84861-5-RR against Human RIG-1/DDX58 were performed. The affinity constant is below 1 pM.

RIG-1/DDX58 Recombinant antibody

RIG-1/DDX58 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84861-5-RR

产品说明书

CloneNo. 242424E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

DDX58, RIG-I, 242424E7, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, THP-1 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84861-5-RR targets RIG-1/DDX58 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RIG-1/DDX58 fusion protein Ag18585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
别名 DDX58, RIG-I, 242424E7, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58
计算分子量 925 aa, 106 kDa
观测分子量 101~106 kDa
GenBank蛋白编号BC132786
基因名称 RIG-1/DDX58
Gene ID (NCBI) 23586
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95786
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

实验方案

Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 84861-5-RRDownload protocol
IF protocol for RIG-1/DDX58 antibody 84861-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols