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验证数据展示

  • WB analysis of A431 using 84861-5-RR (same clone as 84861-5-PBS)

    WB analysis of A431 using 84861-5-RR (same clone as 84861-5-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84861-5-PBS in a different storage buffer formulation.

  • WB analysis using 84861-5-RR (same clone as 84861-5-PBS)

    WB analysis using 84861-5-RR (same clone as 84861-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84861-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84861-5-RR (same clone as 84861-5-PBS)

    IF Staining of A431 using 84861-5-RR (same clone as 84861-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RIG-1/DDX58 antibody (84861-5-RR, Clone: 242424E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84861-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84861-5-RR

    Affinity and Kinetic Characterization of 84861-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84861-5-RR against Human RIG-1/DDX58 were performed. The affinity constant is below 1 pM.

RIG-1/DDX58 Recombinant antibody, PBS Only

RIG-1/DDX58 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84861-5-PBS

产品说明书

CloneNo. 242424E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

DDX58, RIG-I, 242424E7, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84861-5-PBS targets RIG-1/DDX58 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 RIG-1/DDX58 fusion protein Ag18585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
别名 DDX58, RIG-I, 242424E7, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58
计算分子量 925 aa, 106 kDa
观测分子量 101~106 kDa
GenBank蛋白编号BC132786
基因名称 RIG-1/DDX58
Gene ID (NCBI) 23586
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95786
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.