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验证数据展示

  • WB analysis using 82996-4-RR

    WB analysis using 82996-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 82996-4-RR

    WB analysis of HepG2 using 82996-4-RR

    WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells.

  • IHC staining of human ovarian cancer using 82996-4-RR

    IHC staining of human ovarian cancer using 82996-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82996-4-RR

    Affinity and Kinetic Characterization of 82996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

RILP Recombinant antibody

RILP Recombinant Antibody for WB, IHC, ELISA
Cat No. 82996-4-RR
CloneNo. 230208G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Rab-interacting lysosomal protein, PP10141, FLJ31193, 230208G2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82996-4-RR targets RILP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RILP fusion protein Ag4472 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.04 x 10-11M
KOff=2.33 x 10-5M
KOn=2.05 x 106M
Full Name Rab interacting lysosomal protein
Synonyms Rab-interacting lysosomal protein, PP10141, FLJ31193, 230208G2
Calculated Molecular Weight 401 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC031621
Gene Symbol RILP
Gene ID (NCBI) 83547
RRIDAB_3670743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96NA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RILP antibody 82996-4-RRDownload protocol
IHC protocol for RILP antibody 82996-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols