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验证数据展示

  • WB analysis using 82996-4-RR (same clone as 82996-4-PBS)

    WB analysis using 82996-4-RR (same clone as 82996-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82996-4-RR (same clone as 82996-4-PBS)

    WB analysis of HepG2 using 82996-4-RR (same clone as 82996-4-PBS)

    WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

    IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00025-1

    Cytometric bead array standard curve of MP00025-1

    Cytometric bead array standard curve of MP00025-1, RILP Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82996-4-PBS. Detection antibody: 82996-3-PBS. Standard: Ag4472. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 82996-4-RR

    Affinity and Kinetic Characterization of 82996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

RILP Recombinant antibody, PBS Only (Capture)

RILP Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 82996-4-PBS

产品说明书

CloneNo. 230208G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Rab-interacting lysosomal protein, PP10141, FLJ31193, 230208G2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82996-4-PBS targets RILP as part of a matched antibody pair:

MP00025-1: 82996-4-PBS capture and 82996-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RILP fusion protein Ag4472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=1.04 x 10-11M
KOff=2.33 x 10-5M
KOn=2.05 x 106M
全称 Rab interacting lysosomal protein
别名 Rab-interacting lysosomal protein, PP10141, FLJ31193, 230208G2
计算分子量 401 aa, 41 kDa
观测分子量 50 kDa
GenBank蛋白编号BC031621
基因名称 RILP
Gene ID (NCBI) 83547
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96NA2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C