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验证数据展示

  • WB analysis using 83220-6-RR

    WB analysis using 83220-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83220-6-RR (RILPL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83220-6-RR

    WB analysis of HepG2 using 83220-6-RR

    WB result of RILPL1 antibody (83220-6-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 83220-6-RR

    Affinity and Kinetic Characterization of 83220-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83220-6-RR against Human RILPL1 were performed. The affinity constant is 1.97 nM.

  • Featured Product
  • KD/KO验证

RILPL1 Recombinant antibody

RILPL1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83220-6-RR

产品说明书

CloneNo. 230515D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, A549 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83220-6-RR targets RILPL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RILPL1 fusion protein Ag10192 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rab interacting lysosomal protein-like 1
别名 RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378
计算分子量 252 aa, 29 kDa
观测分子量 50 kDa
GenBank蛋白编号BC080626
基因名称 RILPL1
Gene ID (NCBI) 353116
RRIDAB_3670905
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5EBL4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein localization in the primary cilium. Rilpl2 was initially identified as up-regulated in ciliating mouse tracheal epithelial cells. Rilpl1 and Rilpl2 both localize to the primary cilium and centrosome, Rilpl1 specifically to the distal end of the mother centriole.(PMID:23264467)

实验方案

Product Specific Protocols
WB protocol for RILPL1 antibody 83220-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols