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RIT2 Recombinant antibody, PBS Only (Detector)

RIT2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241916A7

Cat no : 84520-1-PBS

Print datasheet

Synonyms

Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2

验证数据展示

  • WB analysis using 84520-1-RR (same clone as 84520-1-PBS)

    WB analysis using 84520-1-RR (same clone as 84520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84520-1-RR (RIT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84520-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01369-1

    Cytometric bead array standard curve of MP01369-1

    Cytometric bead array standard curve of MP01369-1, RIT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84520-2-PBS. Detection antibody: 84520-1-PBS. Standard: Ag3160. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84520-1-RR

    Affinity and Kinetic Characterization of 84520-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84520-1-RR against Human RIT2 were performed. The affinity constant is below 1 pM.

产品信息

84520-1-PBS targets RIT2 as part of a matched antibody pair:

MP01369-1: 84520-2-PBS capture and 84520-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RIT2 fusion protein Ag3160 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Ras-like without CAAX 2
Synonyms Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC018060
Gene Symbol RIT2
Gene ID (NCBI) 6014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).