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RIT2 Recombinant antibody

RIT2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241916A7

Cat no : 84520-1-RR

Print datasheet

Synonyms

Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2

验证数据展示

  • WB analysis using 84520-1-RR

    WB analysis using 84520-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84520-1-RR (RIT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84520-1-RR

    Affinity and Kinetic Characterization of 84520-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84520-1-RR against Human RIT2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tssue, fetal human brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84520-1-RR targets RIT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RIT2 fusion protein Ag3160 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Ras-like without CAAX 2
Synonyms Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC018060
Gene Symbol RIT2
Gene ID (NCBI) 6014
RRIDAB_3672027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99578
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).

实验方案

Product Specific Protocols
WB protocol for RIT2 antibody 84520-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols