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  • Featured Product
  • KD/KO Validated

RNASEH1 Recombinant antibody, PBS Only

RNASEH1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C12

Cat no : 82771-1-PBS

Print datasheet

Synonyms

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

验证数据展示

  • WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82771-1-RR

    Affinity and Kinetic Characterization of 82771-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

产品信息

82771-1-PBS targets RNASEH1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RNASEH1 fusion protein Ag7990 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribonuclease H1
Synonyms RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002973
Gene Symbol RNASEH1
Gene ID (NCBI) 246243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60930
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].