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验证数据展示

  • WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

    WB result of RNASEH1 antibody (82771-1-RR; 1:15000; room temperature for 1.5 hours) with wild-type and RNASEH1 knockout HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82771-1-RR (same clone as 82771-1-PBS)

    IHC staining of human ovarian cancer using 82771-1-RR (same clone as 82771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82771-1-RR (RNASEH1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82771-1-RR

    Affinity and Kinetic Characterization of 82771-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

RNASEH1 Recombinant antibody, PBS Only

RNASEH1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82771-1-PBS

产品说明书

CloneNo. 2C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82771-1-PBS targets RNASEH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 RNASEH1 fusion protein Ag7990 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribonuclease H1
别名 RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC002973
基因名称 RNASEH1
Gene ID (NCBI) 246243
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60930
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].