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RNASEH1 Recombinant antibody

RNASEH1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C12

Cat no : 82771-1-RR

Print datasheet

Synonyms

H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1

验证数据展示

  • WB analysis using 82771-1-RR

    WB analysis using 82771-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82771-1-RR

    WB analysis of HeLa using 82771-1-RR

    WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells.

  • Affinity and Kinetic Characterization of 82771-1-RR

    Affinity and Kinetic Characterization of 82771-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, U2OS cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82771-1-RR targets RNASEH1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen RNASEH1 fusion protein Ag7990 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribonuclease H1
Synonyms H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002973
Gene Symbol RNASEH1
Gene ID (NCBI) 246243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

实验方案

Product Specific Protocols
WB protocol for RNASEH1 antibody 82771-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols