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  • Featured Product
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RNASEH1 Recombinant antibody

RNASEH1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C12

Cat no : 82771-1-RR

Print datasheet

Synonyms

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

验证数据展示

  • WB analysis using 82771-1-RR

    WB analysis using 82771-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82771-1-RR

    WB analysis of HeLa using 82771-1-RR

    WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells.

  • IHC staining of human ovarian cancer using 82771-1-RR

    IHC staining of human ovarian cancer using 82771-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82771-1-RR (RNASEH1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82771-1-RR

    Affinity and Kinetic Characterization of 82771-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, U2OS cells, Jurkat cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82771-1-RR targets RNASEH1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RNASEH1 fusion protein Ag7990 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribonuclease H1
Synonyms RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002973
Gene Symbol RNASEH1
Gene ID (NCBI) 246243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

实验方案

Product Specific Protocols
WB protocol for RNASEH1 antibody 82771-1-RRDownload protocol
IHC protocol for RNASEH1 antibody 82771-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols