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RNASEH2B Recombinant antibody, PBS Only

RNASEH2B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241131F1

Cat no : 84097-6-PBS

Print datasheet

Synonyms

ribonuclease H2, subunit B, Ribonuclease H2 subunit B, DLEU8, Aicardi-Goutieres syndrome 2 protein, AGS2

验证数据展示

  • WB analysis using 84097-6-RR (same clone as 84097-6-PBS)

    WB analysis using 84097-6-RR (same clone as 84097-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84097-6-RR (RNASEH2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84097-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84097-6-RR (same clone as 84097-6-PBS)

    IF Staining of HeLa using 84097-6-RR (same clone as 84097-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNASEH2B antibody (84097-6-RR, Clone: 241131F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84097-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84097-6-RR

    Affinity and Kinetic Characterization of 84097-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human RNASEH2B were performed. The affinity constant is below 1 pM.

产品信息

84097-6-PBS targets RNASEH2B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RNASEH2B fusion protein Ag33669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribonuclease H2, subunit B
Synonyms ribonuclease H2, subunit B, Ribonuclease H2 subunit B, DLEU8, Aicardi-Goutieres syndrome 2 protein, AGS2
Calculated Molecular Weight 331 aa, 37 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC036744
Gene Symbol RNASEH2B
Gene ID (NCBI) 79621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5TBB1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.