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验证数据展示

  • IF Staining of transfected cells using 13753-1-AP (same clone as 13753-1-PBS)

    IF Staining of transfected cells using 13753-1-AP (same clone as 13753-1-PBS)

    IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

  • IP experiment of BxPC-3 using 13753-1-AP (same clone as 13753-1-PBS)

    IP experiment of BxPC-3 using 13753-1-AP (same clone as 13753-1-PBS)

    IP result of anti-RNase T2 (IP:13753-1-AP, 4ug; Detection:13753-1-AP 1:300) with BxPC-3 cells lysate 4000ug. This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 13753-1-AP (same clone as 13753-1-PBS)

    IHC staining of mouse spleen using 13753-1-AP (same clone as 13753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RNASET2 antibody included
  • IHC staining of rat small intestine using 13753-1-AP (same clone as 13753-1-PBS)

    IHC staining of rat small intestine using 13753-1-AP (same clone as 13753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RNASET2 antibody included

RNASET2 Polyclonal antibody, PBS Only

RNASET2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13753-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IP, Indirect ELISA

别名

RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RNASET2 Ready-To-Use IHC Kit. RNASET2 primary antibody included.

产品信息

13753-1-PBS targets RNASET2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RNASET2 fusion protein Ag4695 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonuclease T2
别名 RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19
计算分子量 256 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC039713
基因名称 RNase T2
Gene ID (NCBI) 8635
RRIDAB_2285339
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00584
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).