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RNF135 Polyclonal antibody

RNF135 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25061-1-AP

Print datasheet

Synonyms

RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27

验证数据展示

  • WB analysis of mouse kidney using 25061-1-AP

    WB analysis of mouse kidney using 25061-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 25061-1-AP

    IHC staining of human kidney using 25061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 25061-1-AP

    IHC staining of human kidney using 25061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of PC-12 using 25061-1-AP

    IF Staining of PC-12 using 25061-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using RNF135 antibody (25061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 25061-1-AP

    WB analysis using 25061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 25061-1-AP

    IHC staining of human stomach using 25061-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25061-1-AP (RNF135 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, U-87 MG cells
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25061-1-AP targets RNF135 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RNF135 fusion protein Ag18821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 135
Synonyms RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC005084
Gene Symbol RNF135
Gene ID (NCBI) 84282
RRIDAB_2879879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.

实验方案

Product Specific Protocols
WB protocol for RNF135 antibody 25061-1-APDownload protocol
IHC protocol for RNF135 antibody 25061-1-APDownload protocol
IF protocol for RNF135 antibody 25061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination.

Authors - Yuan-Qin Min
View Article
humanWB

Oncol Lett

Ubiquitination of p21 by E3 ligase RNF135 promotes the proliferation of human glioblastoma cells

Authors - Weiting Gu
View Article
humanWB,IHC

Cancer Rep (Hoboken)

RNF135 Promotes Human Osteosarcoma Cell Growth and Inhibits Apoptosis by Upregulating the PI3K/AKT Pathway

Authors - Bingyao Chen
View Article