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验证数据展示

  • IF Staining of PC-12 using CL488-25061

    IF Staining of PC-12 using CL488-25061

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 RNF135 antibody (CL488-25061) at dilution of 1:200.

CoraLite® Plus 488-conjugated RNF135 Polyclonal antibody

RNF135 Polyclonal Antibody for IF/ICC
Cat No. CL488-25061

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 25061-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25061 targets RNF135 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen RNF135 fusion protein Ag18821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 135
Synonyms RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC005084
Gene Symbol RNF135
Gene ID (NCBI) 84282
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUD6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RNF135 antibody CL488-25061Download protocol
Standard Protocols
Click here to view our Standard Protocols