验证数据展示
经过测试的应用
| Positive IF/ICC detected in | PC-12 cells |
For other applications, we recommend the unconjugated version of this antibody, 25061-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-25061 targets RNF135 in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | RNF135 fusion protein Ag18821 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 135 |
| 别名 | RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27 |
| 计算分子量 | 432 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC005084 |
| 基因名称 | RNF135 |
| Gene ID (NCBI) | 84282 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IUD6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RNF135 antibody CL488-25061 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |