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验证数据展示

  • WB analysis using 84793-5-RR (same clone as 84793-5-PBS)

    WB analysis using 84793-5-RR (same clone as 84793-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84793-5-RR (RNF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 84793-5-RR (same clone as 84793-5-PBS)

    WB analysis of HEK-293 using 84793-5-RR (same clone as 84793-5-PBS)

    WB result of RNF2 antibody (84793-5-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 84793-5-RR (same clone as 84793-5-PBS)

    IF Staining of U2OS using 84793-5-RR (same clone as 84793-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RNF2 antibody (84793-5-RR, Clone: 242181G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84793-5-RR (same clone as 84793-5-PBS)

    FC experiment of HepG2 using 84793-5-RR (same clone as 84793-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNF2 Recombinant antibody (84793-5-RR, Clone:242181G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84793-5-RR

    Affinity and Kinetic Characterization of 84793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84793-5-RR against Human RNF2 were performed. The affinity constant is 0.251 nM.

  • Featured Product
  • KD/KO验证

RNF2 Recombinant antibody, PBS Only

RNF2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84793-5-PBS

产品说明书

CloneNo. 242181G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1, BAP 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84793-5-PBS targets RNF2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RNF2 fusion protein Ag8900 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 2
别名 EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1, BAP 1
计算分子量 336 aa, 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC012583
基因名称 RNF2
Gene ID (NCBI) 6045
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ99496
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.