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验证数据展示

  • WB analysis of SW480 using 83658-2-RR

    WB analysis of SW480 using 83658-2-RR

    SW480 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Caco-2 using 83658-2-RR

    WB analysis of Caco-2 using 83658-2-RR

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of SW480 using 83658-2-RR

    IP experiment of SW480 using 83658-2-RR

    IP result of anti-RNF216 (IP:83658-2-RR, 4ug; Detection:83658-2-RR 1:1000) with SW480 cells lysate 1280 ug.

  • FC experiment of A431 using 83658-2-RR

    FC experiment of A431 using 83658-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RNF216 Recombinant antibody (83658-2-RR, Clone:240489G2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83658-2-RR

    Affinity and Kinetic Characterization of 83658-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83658-2-RR against Human RNF216 were performed. The affinity constant is below 1 pM.

RNF216 Recombinant antibody

RNF216 Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 83658-2-RR

产品说明书

CloneNo. 240489G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF216

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSW480 cells, Caco-2 cells
Positive IP detected inSW480 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83658-2-RR targets RNF216 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 RNF216 fusion protein Ag0238 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 216
别名 Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF216
计算分子量 48 kDa
观测分子量~130 kDa
GenBank蛋白编号BC000787
基因名称 RNF216
Gene ID (NCBI) 54476
RRIDAB_3671266
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NWF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively. Western analysis detected the ~130 kDa band as indicated in literature (PMID: 21270397).

实验方案

Product Specific Protocols
WB protocol for RNF216 antibody 83658-2-RRDownload protocol
IP protocol for RNF216 antibody 83658-2-RRDownload protocol
FC protocol for RNF216 antibody 83658-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols