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RNF216 Recombinant antibody, PBS Only (Capture)

RNF216 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240489G2

Cat no : 83658-2-PBS

Print datasheet

Synonyms

Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF216

验证数据展示

  • WB analysis of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    WB analysis of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • WB analysis of Caco-2 using 83658-2-RR (same clone as 83658-2-PBS)

    WB analysis of Caco-2 using 83658-2-RR (same clone as 83658-2-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • IP experiment of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    IP experiment of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    IP result of anti-RNF216 (IP:83658-2-RR, 4ug; Detection:83658-2-RR 1:1000) with SW480 cells lysate 1280 ug. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83658-2-RR (same clone as 83658-2-PBS)

    FC experiment of A431 using 83658-2-RR (same clone as 83658-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RNF216 Recombinant antibody (83658-2-RR, Clone:240489G2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00643-1

    Cytometric bead array standard curve of MP00643-1

    Cytometric bead array standard curve of MP00643-1, RNF216 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83658-2-PBS. Detection antibody: 83658-5-PBS. Standard: Ag0238. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83658-2-RR

    Affinity and Kinetic Characterization of 83658-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83658-2-RR against Human RNF216 were performed. The affinity constant is below 1 pM.

产品信息

83658-2-PBS targets RNF216 as part of a matched antibody pair:

MP00643-1: 83658-2-PBS capture and 83658-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RNF216 fusion protein Ag0238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 216
Synonyms Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF216
Calculated Molecular Weight 48 kDa
Observed Molecular Weight~130 kDa
GenBank Accession NumberBC000787
Gene Symbol RNF216
Gene ID (NCBI) 54476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NWF9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively. Western analysis detected the ~130 kDa band as indicated in literature (PMID: 21270397).