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RNF31 Recombinant antibody, PBS Only (Capture)

RNF31 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230446H1

Cat no : 83137-3-PBS

Print datasheet

Synonyms

HOIP, HOIL 1 interacting protein, FLJ23501, FLJ10111, EC:2.3.2.31

验证数据展示

  • IF Staining of HepG2 using 83137-3-RR (same clone as 83137-3-PBS)

    IF Staining of HepG2 using 83137-3-RR (same clone as 83137-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 83137-3-RR (same clone as 83137-3-PBS)

    FC experiment of PC-3 using 83137-3-RR (same clone as 83137-3-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00285-1

    Cytometric bead array standard curve of MP00285-1

    Cytometric bead array standard curve of MP00285-1, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-1-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00285-2

    Cytometric bead array standard curve of MP00285-2

    Cytometric bead array standard curve of MP00285-2, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-2-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83137-3-RR

    Affinity and Kinetic Characterization of 83137-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

产品信息

83137-3-PBS targets RNF31 as part of a matched antibody pair:

MP00285-1: 83137-3-PBS capture and 83137-1-PBS detection (validated in Cytometric bead array)

MP00285-2: 83137-3-PBS capture and 83137-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen RNF31 fusion protein Ag34505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=5.82 x 10-10M
KOff=5.93 x 10-5M
KOn=1.02 x 105M
Full Name ring finger protein 31
Synonyms HOIP, HOIL 1 interacting protein, FLJ23501, FLJ10111, EC:2.3.2.31
Calculated Molecular Weight 1072 aa, 120 kDa
GenBank Accession NumberBC017376
Gene Symbol RNF31
Gene ID (NCBI) 55072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96EP0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C