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RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Recombinant Antibody for IF/ICC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

230446H1

Cat No : 83137-3-RR

Print datasheet

Synonyms

FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA

验证数据展示

  • IF Staining of HepG2 using 83137-3-RR

    IF Staining of HepG2 using 83137-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of PC-3 using 83137-3-RR

    FC experiment of PC-3 using 83137-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity experiments of 83137-3-RR

    Affinity experiments of 83137-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83137-3-RR targets RNF31 / HOIP in IF, ELISA, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, ELISA, FC (Intra) Application Description
Tested Reactivity Human
Immunogen RNF31 / HOIP fusion protein Ag34505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=5.82 x 10-10M
KOff=5.93 x 10-5M
KOn=1.02 x 105M
Full Name ring finger protein 31
Synonyms FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA
Calculated Molecular Weight 1072 aa, 120 kDa
GenBank Accession NumberBC017376
Gene Symbol RNF31
Gene ID (NCBI) 55072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96EP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RNF31 / HOIP antibody 83137-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols