• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of MCF-7 using 84770-1-RR (same clone as 84770-1-PBS)

    FC experiment of MCF-7 using 84770-1-RR (same clone as 84770-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RNF4 Recombinant antibody (84770-1-RR, Clone:242197A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84770-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84770-1-RR (same clone as 84770-1-PBS)

    IF Staining of HepG2 using 84770-1-RR (same clone as 84770-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF4 antibody (84770-1-RR, Clone: 242197A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84770-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01553-1

    Cytometric bead array standard curve of MP01553-1

    Cytometric bead array standard curve of MP01553-1, RNF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84770-1-PBS. Detection antibody: 84770-2-PBS. Standard: Ag12115. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84770-1-RR

    Affinity and Kinetic Characterization of 84770-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84770-1-RR against Human RNF4 were performed. The affinity constant is 17.3 nM.

RNF4 Recombinant antibody, PBS Only (Capture)

RNF4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84770-1-PBS

产品说明书

CloneNo. 242197A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4, E3 ubiquitin ligase RNF4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy RNF4 Ready-To-Use IHC Kit. RNF4 primary antibody included.

产品信息

84770-1-PBS targets RNF4 as part of a matched antibody pair:

MP01553-1: 84770-1-PBS capture and 84770-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 RNF4 fusion protein Ag12115 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 4
别名 RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4, E3 ubiquitin ligase RNF4
计算分子量 190 aa, 21 kDa
GenBank蛋白编号BC031935
基因名称 RNF4
Gene ID (NCBI) 6047
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78317
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220].