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  • Featured Product
  • KD/KO Validated

RNF6 Polyclonal antibody, PBS Only

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20437-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20437-1-AP (same clone as 20437-1-PBS)

    IF Staining of HepG2 using 20437-1-AP (same clone as 20437-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using RNF6 antibody 20437-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RNF6 Ready-To-Use IHC Kit. RNF6 primary antibody included.

产品信息

20437-1-PBS targets RNF6 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen RNF6 fusion protein Ag14209 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein (C3H2C3 type) 6
Synonyms
Calculated Molecular Weight 685 aa, 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC034688
Gene Symbol RNF6
Gene ID (NCBI) 6049
RRIDAB_10666853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].