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  • Featured Product
  • KD/KO Validated

ROCK1 Polyclonal antibody, PBS Only

ROCK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21850-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 21850-1-AP (same clone as 21850-1-PBS)

    WB analysis using 21850-1-AP (same clone as 21850-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

    WB analysis of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

    WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

    IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

    IF Staining of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (21850-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

产品信息

21850-1-PBS targets ROCK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ROCK1 fusion protein Ag16317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 1
Synonyms
Calculated Molecular Weight 1354 aa, 158 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
RRIDAB_10953526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13464
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.