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ROCK1 Polyclonal antibody
ROCK1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
IF, IHC, WB, ELISA
Cat No : 21850-1-AP
|Positive WB detected in||A431 cells, HeLa cells, HEK-293T cells, C6 cells, Jurkat cells, NIH/3T3 cells, Ramos cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21850-1-AP is ROCK1 Fusion Protein expressed in E. coli.
|Tested Applications||IF, IHC, WB, ELISA|
|Cited Applications||IF, IHC, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Immunogen||ROCK1 fusion protein Ag16317 种属同源性预测|
|Host / Isotype||Rabbit / IgG|
|Full Name||Rho-associated, coiled-coil containing protein kinase 1|
|Synonyms||p160 ROCK 1, P160ROCK, PRO0435, ROCK1|
|Calculated molecular weight||1354 aa, 158 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||BC113114|
|Gene ID (NCBI)||6093|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
|Product Specific Protocols|
|WB protocol for ROCK1 antibody 21850-1-AP||Download protocol|
|IHC protocol for ROCK1 antibody 21850-1-AP||Download protocol|
|IF protocol for ROCK1 antibody 21850-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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