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ROCK1 Recombinant antibody, PBS Only

ROCK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230423C6

Cat no : 83166-5-PBS

Print datasheet

Synonyms

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

验证数据展示

  • WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

    WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (83166-5-RR, Clone: 230423C6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    FC experiment of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ROCK1 Recombinant antibody (83166-5-RR, Clone:230423C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83166-5-RR

    Affinity and Kinetic Characterization of 83166-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83166-5-RR against Human ROCK1 were performed. The affinity constant is 0.401 nM.

产品信息

The immunogen of 83166-5-PBS is ROCK1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ROCK1 fusion protein Ag16317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 1
Synonyms Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1
Calculated Molecular Weight 1354 aa, 158 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13464
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C