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0
Conjugate
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验证数据展示

  • WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

    WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83166-5-RR (same clone as 83166-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83166-5-RR (same clone as 83166-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83166-5-RR (ROCK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (83166-5-RR, Clone: 230423C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    FC experiment of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ROCK1 Recombinant antibody (83166-5-RR, Clone:230423C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83166-5-RR

    Affinity and Kinetic Characterization of 83166-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83166-5-RR against Human ROCK1 were performed. The affinity constant is 0.401 nM.

ROCK1 Recombinant antibody, PBS Only

ROCK1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83166-5-PBS

产品说明书

CloneNo. 230423C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83166-5-PBS targets ROCK1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ROCK1 fusion protein Ag16317 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho-associated, coiled-coil containing protein kinase 1
别名 Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1
计算分子量 1354 aa, 158 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC113114
基因名称 ROCK1
Gene ID (NCBI) 6093
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13464
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.