ROCK1 Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230423C6

Cat No : 83166-5-PBS

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1

WB analysis using 83166-5-RR (same clone as 83166-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.
IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (83166-5-RR, Clone: 230423C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.
Affinity experiments of 83166-5-RR
Biolayer interferometry (BLl) kinetic assays of 83166-5-RR against Human ROCK1 were performed. The affinity constant is 0.401 nM.

Size

The immunogen of 83166-5-PBS is ROCK1 Fusion Protein expressed in E. coli.

Tested Applications	WB, IF, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	ROCK1 fusion protein Ag16317 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	Rho-associated, coiled-coil containing protein kinase 1
Synonyms	p160 ROCK 1, P160ROCK, PRO0435, ROCK1
Calculated Molecular Weight	1354 aa, 158 kDa
GenBank Accession Number	BC113114
Gene Symbol	ROCK1
Gene ID (NCBI)	6093
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q13464
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.