ROCK1 Recombinant antibody
ROCK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230423C6
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, NIH/3T3 cells, HeLa cells, A431 cells, RAW 264.7 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83166-5-RR is ROCK1 Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | Human, mouse |
Immunogen | ROCK1 fusion protein Ag16317 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | Rho-associated, coiled-coil containing protein kinase 1 |
Synonyms | Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1 |
Calculated Molecular Weight | 1354 aa, 158 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | BC113114 |
Gene Symbol | ROCK1 |
Gene ID (NCBI) | 6093 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q13464 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ROCK1 antibody 83166-5-RR | Download protocol |
IF protocol for ROCK1 antibody 83166-5-RR | Download protocol |
FC protocol for ROCK1 antibody 83166-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |