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验证数据展示

  • WB analysis using CL594-66633

    WB analysis using CL594-66633

    HeLa cells were subjected to SDS PAGE followed by western blot with CL594-66633 (ROCK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ROCK2 Monoclonal antibody

ROCK2 Monoclonal Antibody for WB
Cat No. CL594-66633

Print datasheet

CloneNo. 1C7B8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

WB

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66633-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66633 targets ROCK2 in WB applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen ROCK2 fusion protein Ag16330 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 2
Synonyms KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2
Calculated Molecular Weight 77 kDa, 161 kDa
Observed Molecular Weight 161 kDa
GenBank Accession NumberBC111801
Gene Symbol ROCK2
Gene ID (NCBI) 9475
RRIDAB_2920017
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75116
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

实验方案

Product Specific Protocols
WB protocol for CL594 ROCK2 antibody CL594-66633Download protocol
Standard Protocols
Click here to view our Standard Protocols