验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66633-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66633 targets ROCK2 in WB applications and shows reactivity with Human, Rat, Mouse samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, Rat, Mouse |
| 免疫原 | ROCK2 fusion protein Ag16330 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Rho-associated, coiled-coil containing protein kinase 2 |
| 别名 | KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2 |
| 计算分子量 | 77 kDa, 161 kDa |
| 观测分子量 | 161 kDa |
| GenBank蛋白编号 | BC111801 |
| 基因名称 | ROCK2 |
| Gene ID (NCBI) | 9475 |
| RRID | AB_2920017 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75116 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 ROCK2 antibody CL594-66633 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |