验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human kidney tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ROGDI Ready-To-Use IHC Kit. ROGDI primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17047-1-AP targets ROGDI in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, Drosophila |
| 免疫原 | ROGDI fusion protein Ag10688 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | rogdi homolog (Drosophila) |
| 别名 | Protein rogdi homolog, ROGDI, rogdi homolog (Drosophila) |
| 计算分子量 | 287 aa, 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC012901 |
| 基因名称 | ROGDI |
| Gene ID (NCBI) | 79641 |
| RRID | AB_2182328 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZN7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROGDI antibody 17047-1-AP | Download protocol |
| IHC protocol for ROGDI antibody 17047-1-AP | Download protocol |
| IP protocol for ROGDI antibody 17047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome. | ||
Sci Rep The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein. | ||
Cancer Biol Ther Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.
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