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- KD/KO Validated
ROGDI Polyclonal antibody
ROGDI Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human kidney tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ROGDI Ready-To-Use IHC Kit. ROGDI primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17047-1-AP targets ROGDI in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, Drosophila |
| Immunogen | ROGDI fusion protein Ag10688 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | rogdi homolog (Drosophila) |
| Synonyms | Protein rogdi homolog, ROGDI, rogdi homolog (Drosophila) |
| Calculated Molecular Weight | 287 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC012901 |
| Gene Symbol | ROGDI |
| Gene ID (NCBI) | 79641 |
| RRID | AB_2182328 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROGDI antibody 17047-1-AP | Download protocol |
| IHC protocol for ROGDI antibody 17047-1-AP | Download protocol |
| IP protocol for ROGDI antibody 17047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome. | ||
Sci Rep The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein. | ||
Cancer Biol Ther Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.
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