RON, MST1R Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8D1

Cat no : 67954-1-PBS

Synonyms

MST1R, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor, EC:2.7.10.1, CDw136

WB analysis of BxPC-3 using 67954-1-Ig (same clone as 67954-1-PBS)
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 67954-1-Ig (same clone as 67954-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67954-1-PBS in a different storage buffer formulation.

Size

67954-1-PBS targets RON, MST1R in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications	WB, Indirect ELISA Application Description
Tested Reactivity	human
Immunogen	RON, MST1R fusion protein Ag28289 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms	MST1R, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor, EC:2.7.10.1, CDw136
Calculated Molecular Weight	152 kDa
Observed Molecular Weight	150-185 kDa
GenBank Accession Number	BQ936677 NM_002447
Gene Symbol	RON
Gene ID (NCBI)	4486
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q04912
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C