• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82930-1-RR

    WB analysis using 82930-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82930-1-RR (RORA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82930-1-RR

    IF Staining of HepG2 using 82930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of rat cerebellum using 82930-1-RR

    IHC staining of rat cerebellum using 82930-1-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82930-1-RR (RORA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 82930-1-RR

    IF Staining of rat brain using 82930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using RORA antibody (82930-1-RR, Clone: 230217G6 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 82930-1-RR

    FC experiment of HeLa using 82930-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RORA Recombinant antibody (82930-1-RR, Clone:230217G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

RORA Recombinant antibody

RORA Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 82930-1-RR

产品说明书

CloneNo. 230217G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, L02 cells, HuH-7 cells, PC-3 cells
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:175-1:700
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82930-1-RR targets RORA in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RORA fusion protein Ag0944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAR-related orphan receptor A
别名 Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1
计算分子量 59 kDa
观测分子量60-66 kDa
GenBank蛋白编号BC008831
基因名称 RORA
Gene ID (NCBI) 6095
RRIDAB_3670668
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35398
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

实验方案

Product Specific Protocols
WB protocol for RORA antibody 82930-1-RRDownload protocol
IHC protocol for RORA antibody 82930-1-RRDownload protocol
IF protocol for RORA antibody 82930-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols