RORA Recombinant antibody
RORA Recombinant Antibody for WB, IF/ICC, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat
Applications
WB, IF/ICC, FC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
230217G6
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HepG2 cells, L02 cells, HuH-7 cells, PC-3 cells |
Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:150-1:600 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82930-1-RR targets RORA in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Rat samples.
Tested Applications | WB, IF/ICC, FC, IHC, ELISA Application Description |
Tested Reactivity | Human, Rat |
Immunogen | RORA fusion protein Ag0944 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | RAR-related orphan receptor A |
Synonyms | DKFZp686M2414, NR1F1, Nuclear receptor ROR alpha, Nuclear receptor RZR alpha, RAR related orphan receptor A, ROR1, ROR2, ROR3, RORA, RZR ALPHA, RZRA |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 60-66 kDa |
GenBank Accession Number | BC008831 |
Gene Symbol | RORA |
Gene ID (NCBI) | 6095 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35398 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RORA antibody 82930-1-RR | Download protocol |
IHC protocol for RORA antibody 82930-1-RR | Download protocol |
IF protocol for RORA antibody 82930-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |