验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82930 targets RORA in IF/ICC applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | RORA fusion protein Ag0944 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RAR-related orphan receptor A |
| 别名 | Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1 |
| 计算分子量 | 59 kDa |
| 观测分子量 | 60-66 kDa |
| GenBank蛋白编号 | BC008831 |
| 基因名称 | RORA |
| Gene ID (NCBI) | 6095 |
| RRID | AB_3673140 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35398 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RORA antibody CL488-82930 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |