验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82930 targets RORA in IF/ICC applications and shows reactivity with human, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, rat |
| Immunogen | RORA fusion protein Ag0944 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | RAR-related orphan receptor A |
| Synonyms | Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 60-66 kDa |
| GenBank Accession Number | BC008831 |
| Gene Symbol | RORA |
| Gene ID (NCBI) | 6095 |
| RRID | AB_3673140 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35398 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RORA antibody CL488-82930 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |