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CoraLite®555-conjugated RPA2 Monoclonal antibody

RPA2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1B9H11

Cat no : CL555-67999

Print datasheet

Synonyms

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

验证数据展示

  • IF Staining of A431 using CL555-67999

    IF Staining of A431 using CL555-67999

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 RPA2 antibody (CL555-67999, Clone: 1B9H11 ) at dilution of 1:1000.

  • FC experiment of HeLa using CL555-67999

    FC experiment of HeLa using CL555-67999

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human RPA2 (CL555-67999, Clone:1B9H11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67999-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67999 targets RPA2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen RPA2 fusion protein Ag24783 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A2, 32kDa
Synonyms RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15927
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.

实验方案

Product Specific Protocols
IF protocol for CL555 RPA2 antibody CL555-67999Download protocol
Standard Protocols
Click here to view our Standard Protocols