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验证数据展示

  • WB analysis using 68193-1-Ig

    WB analysis using 68193-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 68193-1-Ig

    FC experiment of HeLa using 68193-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug RPAP1 Monoclonal antibody (68193-1-Ig, Clone:2A6H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

RPAP1 Monoclonal antibody

RPAP1 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 68193-1-Ig

产品说明书

CloneNo. 2A6H5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA, FC (Intra)

别名

RPAP 1, RNA polymerase II-associated protein 1, KIAA1403, 2A6H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, mouse brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68193-1-Ig targets RPAP1 in WB, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 RPAP1 fusion protein Ag7856 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA polymerase II associated protein 1
别名 RPAP 1, RNA polymerase II-associated protein 1, KIAA1403, 2A6H5
计算分子量 153 kDa
观测分子量 153 kDa
GenBank蛋白编号BC000246
基因名称 RPAP1
Gene ID (NCBI) 26015
RRIDAB_2935282
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BWH6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.

实验方案

Product Specific Protocols
WB protocol for RPAP1 antibody 68193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols