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验证数据展示

  • WB analysis using 84670-3-RR

    WB analysis using 84670-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84670-3-RR (RPL11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84670-3-RR

    Affinity and Kinetic Characterization of 84670-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84670-3-RR against Human RPL11 were performed. The affinity constant is below 1 pM.

RPL11 Recombinant antibody

RPL11 Recombinant Antibody for WB, ELISA
Cat No. 84670-3-RR

产品说明书

CloneNo. 242147D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

GIG34, DBA7, CLL-associated antigen KW-12, CLL associated antigen KW 12, 60S ribosomal protein L11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, BxPC-3 cells, mouse pancreas tissue, mouse liver tissue, rat liver tissue
Planning an IHC experiment? We recommend our IHCeasy RPL11 Ready-To-Use IHC Kit. RPL11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84670-3-RR targets RPL11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RPL11 fusion protein Ag9356 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L11
别名 GIG34, DBA7, CLL-associated antigen KW-12, CLL associated antigen KW 12, 60S ribosomal protein L11
计算分子量 177 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC018970
基因名称 RPL11
Gene ID (NCBI) 6135
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP62913
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.

实验方案

Product Specific Protocols
WB protocol for RPL11 antibody 84670-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols