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验证数据展示

  • WB analysis using 14536-1-AP

    WB analysis using 14536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 14536-1-AP

    IHC staining of human placenta using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of human placenta using 14536-1-AP

    IHC staining of human placenta using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of human endometrial cancer using 14536-1-AP

    IHC staining of human endometrial cancer using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of human endometrial cancer using 14536-1-AP

    IHC staining of human endometrial cancer using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of mouse brain using 14536-1-AP

    IHC staining of mouse brain using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of mouse stomach using 14536-1-AP

    IHC staining of mouse stomach using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of rat brain using 14536-1-AP

    IHC staining of rat brain using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included
  • IHC staining of rat stomach using 14536-1-AP

    IHC staining of rat stomach using 14536-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14536-1-AP (RPL12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RPL12 antibody included

RPL12 Polyclonal antibody

RPL12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14536-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman placenta tissue, human endometrial cancer tissue, mouse brain tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RPL12 Ready-To-Use IHC Kit. RPL12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14536-1-AP targets RPL12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RPL12 fusion protein Ag6037 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L12
别名 ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12
计算分子量 18 kDa
观测分子量 21 kDa
GenBank蛋白编号BC050644
基因名称 RPL12
Gene ID (NCBI) 6136
RRIDAB_2269722
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30050
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL12 antibody 14536-1-APDownload protocol
IHC protocol for RPL12 antibody 14536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

CDK1 couples proliferation with protein synthesis.

Authors - Katharina Haneke
View Article
humanWB

Sci Rep

RNA G-quadruplex structures control ribosomal protein production

Authors - DhavalVarshney
View Article