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验证数据展示

  • WB analysis of HeLa using 11005-1-AP (same clone as 11005-1-PBS)

    WB analysis of HeLa using 11005-1-AP (same clone as 11005-1-PBS)

    WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells.. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • WB analysis using 11005-1-AP (same clone as 11005-1-PBS)

    WB analysis using 11005-1-AP (same clone as 11005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 11005-1-AP (same clone as 11005-1-PBS)

    IP experiment of mouse kidney using 11005-1-AP (same clone as 11005-1-PBS)

    IP result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11005-1-AP (same clone as 11005-1-PBS)

    IF Staining of HepG2 using 11005-1-AP (same clone as 11005-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

    IHC staining of human prostate cancer using 11005-1-AP (same clone as 11005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11005-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RPL3 Polyclonal antibody, PBS Only

RPL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11005-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11005-1-PBS targets RPL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RPL3 fusion protein Ag1436 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L3
别名
计算分子量 46/27 kDa
观测分子量 46 kDa
GenBank蛋白编号BC012786
基因名称 RPL3
Gene ID (NCBI) 6122
RRIDAB_2181760
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39023
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.