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验证数据展示

  • IHC staining of human placenta using 82815-1-RR (same clone as 82815-1-PBS)

    IHC staining of human placenta using 82815-1-RR (same clone as 82815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82815-1-RR (RPL3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82815-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82815-1-RR (same clone as 82815-1-PBS)

    FC experiment of HepG2 using 82815-1-RR (same clone as 82815-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPL3 Recombinant antibody (82815-1-RR, Clone:3P18) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82815-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82815-1-RR (same clone as 82815-1-PBS)

    IF Staining of HepG2 using 82815-1-RR (same clone as 82815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL3 antibody (82815-1-RR, Clone: 3P18 ) at dilution of 1:240 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82815-1-PBS in a different storage buffer formulation.

RPL3 Recombinant antibody, PBS Only

RPL3 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82815-1-PBS

产品说明书

CloneNo. 3P18

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ribosomal protein L3, OK/SW-cl.32, Large ribosomal subunit protein uL3, HIV-1 TAR RNA-binding protein B, 60S ribosomal protein L3

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82815-1-PBS targets RPL3 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 RPL3 fusion protein Ag1436 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L3
别名 ribosomal protein L3, OK/SW-cl.32, Large ribosomal subunit protein uL3, HIV-1 TAR RNA-binding protein B, 60S ribosomal protein L3
计算分子量 46/27 kDa
GenBank蛋白编号BC012786
基因名称 RPL3
Gene ID (NCBI) 6122
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP39023
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.