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Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 11302-1-AP

Print datasheet

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

验证数据展示

WB analysis using 11302-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 11302-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ribosomal protein L4 antibody (11302-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HeLa using 11302-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Ribosomal protein L4 (11302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of human lymphoma using 11302-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11302-1-AP (Ribosomal protein L4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RPL4 antibody included
WB analysis of HeLa using 11302-1-AP
WB result of Ribosomal protein L4 antibody (11302-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HeLa cells.
IP experiment of HeLa using 11302-1-AP
IP result of anti-Ribosomal protein L4 (IP:11302-1-AP, 4ug; Detection:11302-1-AP 1:6000) with HeLa cells lysate 1050 ug.

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Cat No. 11302-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 34 publications below
IF	See 2 publications below
IP	See 2 publications below
FC	See 1 publications below
RIP	See 1 publications below

产品信息

11302-1-AP targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IF, IP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rice, arabidopsis
Immunogen	Ribosomal protein L4 fusion protein Ag1828 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ribosomal protein L4
Synonyms	RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC009888
Gene Symbol	Ribosomal protein L4
Gene ID (NCBI)	6124
RRID	AB_2181909
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36578
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

实验方案

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 11302-1-AP	Download protocol
IHC protocol for Ribosomal protein L4 antibody 11302-1-AP	Download protocol
IF protocol for Ribosomal protein L4 antibody 11302-1-AP	Download protocol
IP protocol for Ribosomal protein L4 antibody 11302-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	WB	Science The Ccr4-Not complex monitors the translating ribosome for codon optimality. Authors - Robert Buschauer View Article
human	WB	Cell Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate. Authors - Colin Chih-Chien Wu View Article
mouse	IF	Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. Authors - Xiaochen Gai View Article
mouse	WB	Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages Authors - Teodorus Theo Susanto View Article
human	WB	Mol Cell N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response. Authors - Jun Zhou View Article
human	WB	Mol Cell The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation. Authors - Cindy Meyer View Article

Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 11302-1-AP

IF Staining of HeLa using 11302-1-AP

FC experiment of HeLa using 11302-1-AP

IHC staining of human lymphoma using 11302-1-AP

WB analysis of HeLa using 11302-1-AP

IP experiment of HeLa using 11302-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

The Ccr4-Not complex monitors the translating ribosome for codon optimality.

Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.

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