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验证数据展示

  • IF Staining of HepG2 using CL488-11302

    IF Staining of HepG2 using CL488-11302

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Ribosomal protein L4 antibody (CL488-11302) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for IF/ICC
Cat No. CL488-11302

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11302-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11302 targets Ribosomal protein L4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Ribosomal protein L4 fusion protein Ag1828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L4
Synonyms 60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009888
Gene Symbol Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_3083897
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36578
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Ribosomal protein L4 antibody CL488-11302Download protocol
Standard Protocols
Click here to view our Standard Protocols