验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11302-1-AP
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-11302 targets Ribosomal protein L4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Ribosomal protein L4 fusion protein Ag1828 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ribosomal protein L4 |
别名 | 60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4 |
计算分子量 | 48 kDa |
观测分子量 | 48 kDa |
GenBank蛋白编号 | BC009888 |
基因名称 | Ribosomal protein L4 |
Gene ID (NCBI) | 6124 |
RRID | AB_3083897 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P36578 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Ribosomal protein L4 antibody CL488-11302 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |