验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RPL6 Ready-To-Use IHC Kit. RPL6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67729-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67729 targets RPL6 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | RPL6 fusion protein Ag6840 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L6 |
| 别名 | 60S ribosomal protein L6, Neoplasm related protein C140, ribosomal protein L6, RPL6, SHUJUN 2, TAXREB107, TXREB1 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC004138 |
| 基因名称 | RPL6 |
| Gene ID (NCBI) | 6128 |
| RRID | AB_2919530 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02878 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RPL6 antibody CL488-67729 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |