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CoraLite® Plus 488-conjugated RPL6 Monoclonal antibody

RPL6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F6F8

Cat no : CL488-67729

Print datasheet

Synonyms

60S ribosomal protein L6, Neoplasm related protein C140, ribosomal protein L6, RPL6, SHUJUN 2, TAXREB107, TXREB1

验证数据展示

  • IF Staining of HepG2 using CL488-67729

    IF Staining of HepG2 using CL488-67729

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RPL6 antibody (CL488-67729, Clone: 3F6F8 ) at dilution of 1:600.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL6 Ready-To-Use IHC Kit. RPL6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67729-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67729 targets RPL6 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen RPL6 fusion protein Ag6840 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein L6
Synonyms 60S ribosomal protein L6, Neoplasm related protein C140, ribosomal protein L6, RPL6, SHUJUN 2, TAXREB107, TXREB1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC004138
Gene Symbol RPL6
Gene ID (NCBI) 6128
RRIDAB_2919530
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02878
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPL6 antibody CL488-67729Download protocol
Standard Protocols
Click here to view our Standard Protocols