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RPL7L1 Polyclonal antibody

RPL7L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16707-1-AP

Print datasheet

Synonyms

RPL7 L1, Ribosomal protein uL30-like, Large ribosomal subunit protein uL30-like 1, 60S ribosomal protein L7-like 1

验证数据展示

  • WB analysis using 16707-1-AP

    WB analysis using 16707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16707-1-AP

    IP experiment of HepG2 using 16707-1-AP

    IP result of anti-RPL7L1 (IP:16707-1-AP, 4ug; Detection:16707-1-AP 1:1000) with HepG2 cells lysate 2240 ug.

  • WB analysis using 16707-1-AP

    WB analysis using 16707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16707-1-AP

    IHC staining of human liver cancer using 16707-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 16707-1-AP

    IHC staining of human liver cancer using 16707-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 16707-1-AP

    IF Staining of MCF-7 using 16707-1-AP

    Immunofluorescent analysis of MCF-7 cells, using RPL7L1 antibody 16707-1-AP at 1:25 dillution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16707-1-AP targets RPL7L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL7L1 fusion protein Ag10106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L7-like 1
Synonyms RPL7 L1, Ribosomal protein uL30-like, Large ribosomal subunit protein uL30-like 1, 60S ribosomal protein L7-like 1
Calculated Molecular Weight 246 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC058020
Gene Symbol RPL7L1
Gene ID (NCBI) 285855
RRIDAB_1851609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DKI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPL7L1 antibody 16707-1-APDownload protocol
IHC protocol for RPL7L1 antibody 16707-1-APDownload protocol
IF protocol for RPL7L1 antibody 16707-1-APDownload protocol
IP protocol for RPL7L1 antibody 16707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
View Article
mouse

Zygote

Identification of four genes required for mammalian blastocyst formation.

Authors - Maserati Marc M
View Article