RPP30 Recombinant antibody

RPP30 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83481-5-RR

产品说明书

CloneNo. 240468E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2, 240468E4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, U-251 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83481-5-RR targets RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 RPP30 fusion protein Ag8600 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribonuclease P/MRP 30kDa subunit
别名 TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2, 240468E4
计算分子量 268 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC006991
基因名称 RPP30
Gene ID (NCBI) 10556
RRIDAB_3671109
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78346
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.

实验方案

Product Specific Protocols
WB protocol for RPP30 antibody 83481-5-RRDownload protocol
IHC protocol for RPP30 antibody 83481-5-RRDownload protocol
IF protocol for RPP30 antibody 83481-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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