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RPS17 Recombinant antibody

RPS17 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240851H3

Cat no : 83848-4-RR

Print datasheet

Synonyms

RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17

验证数据展示

  • WB analysis using 83848-4-RR

    WB analysis using 83848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83848-4-RR

    IF Staining of U2OS using 83848-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83848-4-RR

    FC experiment of HeLa using 83848-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83848-4-RR

    Affinity and Kinetic Characterization of 83848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83848-4-RR targets RPS17 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RPS17 fusion protein Ag9334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S17
Synonyms RPS17L1, RPS17L, ribosomal protein S17, DBA4, 40S ribosomal protein S17
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC009407
Gene Symbol RPS17
Gene ID (NCBI) 6218
RRIDAB_3671434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08708
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

实验方案

Product Specific Protocols
WB protocol for RPS17 antibody 83848-4-RRDownload protocol
IF protocol for RPS17 antibody 83848-4-RRDownload protocol
FC protocol for RPS17 antibody 83848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols