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验证数据展示

  • IF Staining of HepG2 using 83327-1-RR

    IF Staining of HepG2 using 83327-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS18 antibody (83327-1-RR, Clone: 240004C10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 83327-1-RR

    FC experiment of HepG2 using 83327-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPS18 Recombinant antibody (83327-1-RR, Clone:240004C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83327-1-RR

    Affinity and Kinetic Characterization of 83327-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human RPS18 were performed. The affinity constant is 0.47 nM.

RPS18 Recombinant antibody

RPS18 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83327-1-RR

产品说明书

CloneNo. 240004C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Ke-3, KE3, KE 3, HKE3, D6S218E

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83327-1-RR targets RPS18 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 RPS18 fusion protein Ag12505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S18
别名 Ke-3, KE3, KE 3, HKE3, D6S218E
计算分子量 152 aa, 18 kDa
GenBank蛋白编号BC106063
基因名称 RPS18
Gene ID (NCBI) 6222
RRIDAB_3670992
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP62269
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RPS18 antibody 83327-1-RRDownload protocol
FC protocol for RPS18 antibody 83327-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols