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CoraLite® Plus 488-conjugated RPS18 Recombinant antibody

RPS18 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240004C10

Cat no : CL488-83327

Print datasheet

Synonyms

Ke-3, KE3, KE 3, HKE3, D6S218E

验证数据展示

  • IF Staining of HepG2 using CL488-83327

    IF Staining of HepG2 using CL488-83327

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RPS18 antibody (CL488-83327, Clone: 240004C10 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-83327

    FC experiment of HepG2 using CL488-83327

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rps18 Recombinant Antibody (CL488-83327, Clone:240004C10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83327-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83327 targets RPS18 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen RPS18 fusion protein Ag12505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S18
Synonyms Ke-3, KE3, KE 3, HKE3, D6S218E
Calculated Molecular Weight 152 aa, 18 kDa
GenBank Accession NumberBC106063
Gene Symbol RPS18
Gene ID (NCBI) 6222
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62269
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPS18 antibody CL488-83327Download protocol
FC protocol for CL Plus 488 RPS18 antibody CL488-83327Download protocol
Standard Protocols
Click here to view our Standard Protocols