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CoraLite® Plus 488-conjugated RPS20 Monoclonal antibody

RPS20 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E9B4

Cat no : CL488-68270

Print datasheet

Synonyms

RPS20, Small ribosomal subunit protein uS10, 3E9B4, 40S ribosomal protein S20, ribosomal protein S20

验证数据展示

  • IF Staining of HeLa using CL488-68270

    IF Staining of HeLa using CL488-68270

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RPS20 antibody (CL488-68270, Clone: 3E9B4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68270

    FC experiment of HeLa using CL488-68270

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RPS20 (CL488-68270, Clone:3E9B4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS20 Ready-To-Use IHC Kit. RPS20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68270-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68270 targets RPS20 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen RPS20 fusion protein Ag8275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein S20
Synonyms RPS20, Small ribosomal subunit protein uS10, 3E9B4, 40S ribosomal protein S20, ribosomal protein S20
Calculated Molecular Weight 13 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC007507
Gene Symbol RPS20
Gene ID (NCBI) 6224
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60866
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPS20 antibody CL488-68270Download protocol
FC protocol for CL Plus 488 RPS20 antibody CL488-68270Download protocol
Standard Protocols
Click here to view our Standard Protocols